Running Head: The Golgi proteome and novel putative glycosyltransferases Corresponding Author:

Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.

The Golgi apparatus is the central organelle in the secretory pathway and plays key roles in glycosylation, protein sorting, and secretion in plants. Enzymes involved in the biosynthesis of complex polysaccharides, glycoproteins, and glycolipids are located in this organelle, but the majority of them remain uncharacterized. Here, we studied the Arabidopsis (Arabidopsis thaliana) membrane proteo...

Running head : Proteome profile of hp 1 tomato fruits Corresponding

Ganglioside biosynthesis. Concentration of glycosphingolipid glycosyltransferases in Golgi apparatus from rat liver.

Golgi apparatus fractions from rat liver contained all glycosyltransferases which catalyze the in vitro biosynthesis of N-acetylneuraminylgalactosylglucosylceramide (G&, (Nacetylneuraminyl)2-galactosylglucosylceramide (GD3), N-acetylgalactosaminyl(Nacetylneuraminyl) galactosylglucosylceramide (GMz), galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosylglucosylceramide (GM& and Nacet...

In Silico Analysis of Golgi Glycosyltransferases: A Case Study on the LARGE-Like Protein Family

Glycosylation is one of the major post-translational modification processes essential for expression and function of many proteins. It has been estimated that 1% of the open reading frames of a genome is dedicated to glycosylation. Many different enzymes are involved in glycosylation, such as glycosyltransferases and glycosidases. Traditionally, glycosyltransferases are classified based on thei...

A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi fragmentation by interaction with non-muscle myosin IIA.

The Golgi apparatus undergoes morphological changes under stress or malignant transformation, but the precise mechanisms are not known. We recently showed that non-muscle myosin IIA (NMIIA) binds to the cytoplasmic tail of Core 2 N-acetylglucosaminyltransferase mucus-type (C2GnT-M) and transports it to the endoplasmic reticulum for recycling. Here, we report that Golgi fragmentation induced by ...